Epidermal hypocotyl cells are characterized past a highly structured cortical microtubule array, consisting of bundles of polarized, parallel microtubules that gradually drift across the cortex in a rotary style (Chan et al., 2007). In animal cells, microtubules arise from centrosomes; notwithstanding, plant cells lack centrosomes, and microtubules are idea to self-organize into structured arrays (reviewed in Dixit and Cyr, 2004). In a study using green fluorescent protein (GFP)-tubulin, Murata et al. (2005) demonstrated that new microtubules branch off preexisting microtubules in the cortex of institute cells. However, this capacity for branching seems at odds with the tendency of microtubules to form parallel bundles.

Chan et al. (pages 2298–2306) hypothesized that cortical microtubule arrays persist considering some of the newly formed microtubules grow along the preexisting microtubules on which they form. To test this hypothesis, they analyzed the origin of microtubules in the cortex of Arabidopsis interphase hypocotyl cells using fluorescently tagged Arabidopsis Stop binding 1 protein (Pro35S:AtEB1a-GFP). In contrast with GFP-tubulin, which labels unabridged microtubules and does non readily distinguish between neighboring microtubules, AtEB1a-GFP predominantly labels the fast-growing plus ends of microtubules and thereby allows individual microtubules, of known polarity, to be tracked. Apart from labeling the plus end comets of emerging microtubules, AtEB1a-GFP also labeled the positions on the female parent microtubules from which new microtubules arose. To ostend that the EB1a foci on mother microtubules indeed correspond microtubule-nucleating sites, the researchers transiently coexpressed in Nicotiana benthamiana Pro35S:AtEB1a-GFP and RFP-NEDD1, which labels the centrosome in creature cells (Fant et al., 2009) and the spindle poles in plant mitotic cells (Zeng et al., 2009). Since new microtubules were found to sally from foci labeled with both of these probes, EB1 was considered to exist a true-blue marker of microtubule nucleation.

The authors generated kymographs, which rail the origin and fate of fluorescently labeled microtubules, from sets of time-lapse confocal images. Near new microtubules were found to branch forward, toward the plus stop of the mother microtubule, and branching occurred on both sides of the mother microtubule. Yet, in support of the researchers' hypothesis, 38% (n = 165) of new microtubules grew along the axis of the mother microtubule (run into figure

Kymograph showing a single microtubule (+1) emerging from a stationary focus on the mother microtubule (F; vertical line) and growing along the mother microtubule (0° axis). Bar = 4 μm in the x axis and 98 s in the y axis.

Kymograph showing a single microtubule (+1) emerging from a stationary focus on the female parent microtubule (F; vertical line) and growing along the mother microtubule (0° centrality). Bar = 4 μm in the 10 axis and 98 s in the y axis.

Kymograph showing a single microtubule (+1) emerging from a stationary focus on the mother microtubule (F; vertical line) and growing along the mother microtubule (0° axis). Bar = 4 μm in the x axis and 98 s in the y axis.

Kymograph showing a single microtubule (+ane) emerging from a stationary focus on the mother microtubule (F; vertical line) and growing along the mother microtubule (0° centrality). Bar = 4 μm in the x axis and 98 s in the y centrality.

). Few emerging microtubules grew backward, toward the minus finish of the mother microtubule. Furthermore, new microtubules frequently arose from points where two microtubules crossed, and nearly of these microtubules followed the centrality of one of the preexisting microtubules. These results were confirmed in seedlings expressing ProAtSPR1:GFP-AtSPR1, another mark of microtubule plus ends in plants.

Thus, cortical microtubules were found to persist because a population of new microtubules grows along the tracks of existing microtubules.

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Author notes

© 2009 American Society of Plant Biologists

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